 Objetives
Flow Cytometry Laboratory of IdiPAZ supports the scientific activity contributing to the advance in the research, at present benefit of different research groups of the Institute. The equipment allows for phenotypic analysis of subpopulations, quantification of soluble factors in biological samples and culture supernatants, cell cycle experiments.
Composition
Coordinador:
Kilian Gutiérrez Viñas
Telephone: (+34) 91 207 10 21
Secretary:
Susana García Pérez
Telephone: (+34) 91 207 15 12
Resources
Flow Cytometry Laboratory of IdiPAZ supports the scientific activity contributing to the advance in the research, at present benefit of different research groups of the Institute. The equipment allows for phenotypic analysis of subpopulations, quantification of soluble factors in biological samples and culture supernatants, cell cycle experiments.
The Flow Cytometry Laboratory of IdiPAZ has the following facilities:
• One Beckman Coulter Navios cytometer analyser is a clinical system that delivers analytical excellence by coupling extraordinary sensitivity, resolution and dynamic range with high-speed data collection. With superior detection capabilities, the Navios provides accurate measurements, especially for rare events, which is critical to clinical diagnosis and monitoring. The fully-featured, easy-to-use Navios software provides tools to improve the quality of results as well as optimize workflow efficiencies in a clinical lab.
- This analyser has three lasers; Blue solid state Diode: 488nm, 22mwlaser output, Red solid state Diode: 638nm, 25mwlaser output and Violet solid state Diode: 405nm, 40mw laser output. The Navios can analyse ten colours simultaneously in samples marked with the following fluorochromes:• FL1: FITC, GFP, Cy2, Alexa 488 • FL2: PE, Cy3 • FL3: PI, ECD, PE-TEXAS RED, RED RFD • FL4: PE-Cy5, PerCP, Per-CP5.5, TC 7AAD, SPRD • FL5:PE-Cy7 • FL6: APC, Alexa 647 • FL7: APC-Alexa700, Alexa 700 • FL8: APC-Cy7, APC-Alexa750, APC-Alexa780 • FL9: Pacific Blue, eFluor 450 Cyan, Cyan, Violet RFD • FL10: Pacific Orange, AmCyan
- The Navios is connected to computer using the Navios acquisition and analysis programme.
• Two Becton Dickinson FACSCalibur™ cytometer analysers is a platform allows users to perform both cell analysis and cell sorting in a single benchtop system. The system supports a wide variety of research and clinical applications and is complemented by a broad suite of intuitive software solutions to streamline analysis for a wide range of applications including enumeration of lymphocyte subsets, stem cells, residual white blood cells, and reticulocytes.
- Each analyser has two lasers, a 488nm argon laser and a 635nm solid-state red diode laser. The FACSCalibur ™ can analyse four colours simultaneously in samples marked with the following fluorochromes: • FL1: FITC, Alexa 488 • FL2: PE, PI • FL3: PE-Cy5, PerCP, Per-CP5.5 • FL4: APC, APCCy7, Alexa 647
- The two cytometers are connected to Macintosh G4 OS.9.2.2 computers using the CellQuest™ acquisition and analysis programme, and Macintosh G5 OSX with CellQuestPro™ acquisition and analysis software
• One Becton Dickinson FACSCelesta™ cytometer analyser: It is designed to make multicolored flow cytometry more accessible and allow researchers to benefit from new equipment and reagent technological innovations. These optical and reagent technology parallel advances allow multi-laser equipment to complement with new bright fluorochromes, providing a deeper and more powerful insight into cell analysis.
- This analyser has three lasers: Blue solid state Diode (4 detectors), Violet solid state Diode (6 detectors), and Ultra Violet solid state Diode (2 detectors), with which FACSCelesta™ can analyse twelve colors simultaneously.
Portfolio of services/Prices
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